Biological Safety Cabinets and Their Classifications



An important piece of safety equipment is definitely the Biological Safety Cabinet (BSCs) in which manipulations of infectious microorganisms are performed. Biological safety cabinets are the primary signifies of containment developed for working safely with infectious microorganisms. Horizontal clean benches that direct towards the operator are usually not biological safety cabinets and must not be used for handling infectious, toxic or sensitizing materials. BSCs are created to supply each a clean work environment and protection for employees who work with biological hazards consequently biosafety cabinet's uses vertical laminar airflow to make a barrier to airborne particles, such as microorganisms as vertical laminar air flow supplies protection for both the personnel plus the product. Get a lot more information regarding Biological Safety Cabinets


Biosafety cabinets use the HEPA filtered air for the recirculation over the work region. The HEPA filter removes airborne particles in the air, but doesn't eliminate chemical fumes; therefore Biosafety cabinets must not be confused together with the fume hood. Biosafety cabinets aren't the Fume Hood. Biosafety cabinets are equipped with Ultraviolet lamp that offers protection against contamination, but because the UV light can't penetrate the dust particles, thus another chemical disinfectant, iodophor ought to be use for the cleaning and decontaminating the work region.


Use of biosafety cabinets is suggested for the manipulations of pathogens or antineoplastics that will generate aerosols and for all the airborne transmitted pathogens (which include Brucella abortus, Mycobacterium tuberculosis, and so forth).


You can find 3 classes of biological safety cabinets;


BSCs Class I: an open-front negative pressure cabinet. The exhaust air in the cabinet is filtered by a HEPA filter. This class gives personnel and environmental protection, but not product protection.


BSCs Class II: is definitely an open-front, ventilated cabinet. This cabinet delivers a HEPA-filtered, recirculated mass airflow within the work space. The exhaust air in the cabinet is also filtered by HEPA filters. This class provides personnel, atmosphere and product protection.


BSCs Class III: is actually a entirely enclosed ventilated cabinet of gas-tight construction. Operations inside the Class III cabinet are carried out by way of attached rubber gloves. When in use, the Class III cabinet is maintained via damaging air pressure of at the least 0.5 inches water gauge. Supply air is drawn in to the cabinet by means of HEPA filters. The cabinet exhaust air is filtered by two HEPA filters, installed in series, prior to discharge outside on the facility. The exhaust fan for the Class III cabinet is commonly separate in the exhaust fans on the facility's ventilation system.

Go Back